Journal of Clinical Virology - Volume 82 - Supplement 1

Abstracts / Journal of Clinical Virology 82S (2016) S1–S142

S117

more, for monitoring of intensity, RSV-specific denominators are

needed.

http://dx.doi.org/10.1016/j.jcv.2016.08.234

Abstract no: 253

Presentation at ESCV 2016: Poster 195

Herpes Simplex 1-2 in broncho alveolar fluid: A

5 years retrospective study

Catherine Mengelle

1 ,

∗

, Jacques Izopet

1 , 2

Emmanuel Bories

, Aurélie Lecour

Jean-Michel Mansuy

Department of Virology, Toulouse University

Hospital, Toulouse, France

Department of Physiopathology, Toulouse Purpan,

Unité Inserm U563, Toulouse, France

Aim:

Respiratory viruses are very often detected in pneumo-

nia thanks to uniplex and multiplex real-time PCR techniques.

Moreover viruses that are not primilarly the cause of respiratory

infections may also be detected such as HSV 1-2, varicella zoster

virus and cytomegalovirus.

The aim of our study was to analyze the prevalence of other

respiratory infections among broncho-alveolar lavages (BAL) posi-

tive for HSV 1-2. The clinical outcome of patients according to the

anti HSV 1-2 treatment was also analyzed.

Material and methods:

Data from hospitalized patients

suffering from serious respiratory symptoms and whose broncho-

alveolar lavages were positive for HSV 1-2 by an in-house real-time

PCR were analyzed.

Nucleic acids (NA) had been extracted with the MagNA Pure

96 DNA and Viral NA Small Volume kit

on the MagNA Pure 96

instrument (Roche Molecular Diagnostics, Meylan, France).

Samples had been tested for 16 respiratory viruses (influenza

A and B, parainfluenza 1–4, respiratory syncytial viruses A and

B, human metapneumovirus, coronaviruses 229E, OC43 and NL63,

rhinoviruses, enteroviruses, bocaviruses and adenoviruses) with a

multiplex RT-PCR (Anyplex

II RV16 Detection

, Seegene) on the

CFX96

Real-Time System (Biorad diagnostics).

NA had also been tested for cytomegalovirus, varicella zoster

virus employing amonoplex in-house PCR on the Light Cycler or the

Light Cycler 480

(Roche Molecular Diagnostics, Meylan, France).

Data were analyzed on StataTM software (StataCorp, Texas)

using the exact Fisher test.

Results:

Between 2011 and 2015, 122 (73 males) patients

attending an intensive care unit in the Toulouse University Hos-

pital were selected with a HSV 1-2 positive result (mean age 62;

24–86). 119 samples were HSV-1 and three were HSV-2.

117 had been tested with the Anyplex

II RV16 Detection

Seegene

. All the viruses of the panel had been detected except

parainfluenza 1, 2 and 4, human metapneumovirus and bocavirus;

Influenza viruses were the most detected (

= 13), followed by rhi-

novirus (

= 6), respiratory syncytial virus (

= 5) and adenovirus

(

= 4). 117 samples had been tested for cytomegalovirus (26 posi-

tive), and 90 for varicella zoster virus (negative).

28 among the 78 samples that tested positive for HSV 1-2

during the winter season (November to April) were also positive

for another respiratory virus. During the summer season (May to

October) 44 samples tested positive for HSV 1-2 with only 4 in

coinfection (

< 0.005).

The mortality rate did not differ between the HSV 1-2 positive

patients treated with acyclovir or valacyclovir (

= 57) and those

who were not (

= ns).

Conclusions:

Our results indicate that HSV 1-2 infection is

frequent among patients hospitalized in intensive care unit. Dur-

ing the winter season this infection is linked to other respiratory

viruses.

The apparent clinical inefficiency of anti HSV 1-2 treatment indi-

cates that the presence of the virus is more a witness of a clinically

poor condition rather than a cause of it.

http://dx.doi.org/10.1016/j.jcv.2016.08.235

Abstract no: 255

Presentation at ESCV 2016: Poster 196

Three years (2013–2016) of human respiratory

syncytial virus surveillance at a tertiary hospital

in Catalonia, Spain

Laura Gimferrer

∗

, José Angel Rodrigo,

Cristina Andrés, Isabel Dolores Oriolo,

Maria Gema Codina, Paula Peremiquel,

Maria del Carmen Martin, Francisco Fuentes,

Rosario Saiz, Pilar Alcubilla, Magda Campins,

Tomàs Pumarola, Andrés Antón

Hospital Universitari Vall d’Hebron, Universitat

Autònoma de Barcelona, Spain

Background:

Human respiratory syncytial virus (HRSV) is the

most common respiratory pathogen and the main cause of lower

respiratory tract infections among infants and young children. Its

genome is a lineal single-stranded negative-sense RNA of approx-

imately 15 kb that contains 10 genes encoding 11 proteins. The G

glycoprotein in the viral envelope plays an essential role in the virus

attachment. Antigenic and genetic differences in this protein lead

classify HRSV into two different groups, HRSV-A and HRSV-B. Fur-

thermore, based on the hypervariable region 2 (HVR-2) located in

the C-terminal domain of the G protein several genotypes have

been described. Selection pressure drives G protein to continu-

ously evolve, resulting in the likely replacement of predominant

genotype season by season. In the present study the epidemiology

of HRSV viruses detected in respiratory specimens from patients

attended at the Hospital Universitari Vall d’Hebron in Barcelona

(Spain) during three consecutive years (from 2013 to 2016) has

been described.

Material and methods:

FromOctober 2013 (week 40) to March

2016 (week 20) respiratory specimens frompatientswere collected

for laboratory confirmation of respiratory virus infection using

immunochromatography (Binax Now RSV Card, Allere Scarbor-

ough Inc, USA), immunofluorescence (D

Ultra 8

DFA Respiratory

Virus Screening & Identification Kit, Diagnostic HYBRIDS, USA) or

real-time multiplex RT-PCR (Anyplex II RV16 Detection Kit, See-

gene, Korea) assays. A nucleoprotein-specific real time RT-PCR was

performed to determine HRSV group. In addition, phylogenetic

analyses and molecular characterizations were carried out using

MEGAv5.2 software based on the HVR-2 sequence from a repre-

senting sampling of HRSV per week.

Results:

A total of 16552 specimens were collected, of which

1324 (8.3%) were positive for HRSV. The virus showed a seasonal

pattern of circulation, previous to influenza annual epidemics, with

a maximum detection rates in the weeks 52 or 53 in all three

seasons. Viruses belonging to both HRSV groups were detected:

HRSV-A (662; 50%), HRSV-B (579, 44%), HRSV-A/B co-infection (8;

<1%), and 75 (6%) remained unsubtyped. There was an alterna-

tion in the predominance of HRSV group by season; while HRSV-B

was predominant during the first two seasons, HRSV-A became it

during the third. Based on HVR-2 phylogenetic analyses, HRSV-A

viruses belonged to ON1 genotype (153; 99%), but 2 (1%) to NA1.