Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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S122 Abstracts / Journal of Clinical Virology 82S (2016) S1–S142
Abstract no: 308
Presentation at ESCV 2016: Poster 204
Molecular characterization of respiratory
syncytial virus during 2015–2016 season in
Portugal
P. Cristóvão ∗, D. Pereira , P. Pechirra ,
J. Pereira-Vaz , L. Correia , F. Rodrigues ,
G. Andrade , R. Côrte-Real , Paula Branquinho ,
M.J. Peres , R. Viseu , M.J. Balseiro , P. Mota ,
R. Guiomar
1 National Influenza and Other Respiratory Viruses
Reference Laboratory, Infectious Diseases
Department, National Institute of Health Dr. Ricardo
Jorge, Lisboa, Portugal
2 Laboratory of Molecular Biology, Clinical Pathology
Unit, Centro Hospitalar e Universitário de Coimbra,
Coimbra, Portugal
3 Laboratory of Molecular Biology, Clinical Pathology
Unit, Centro Hospitalar do Funchal, Madeira,
Portugal
4 Laboratory of Molecular Biology, Clinical Pathology
Unit, Centro Hospitalar Lisboa Central, Lisboa,
Portugal
5 Immunology and Molecular Biology Laboratory,
Allergy and Clinical Immunology Department, Centro
Hospitalar de Setúbal, Setúbal, Portugal
6 Pediatrics Department, Centro Hospitalar de
Setúbal, Setúbal, Portugal
7 Microbiology Laboratory, Clinical Pathology
Department, Hospital Senhora da Oliveira,
Guimarães, Portugal
Background: Respiratory syncytial virus (RSV) is one of themost
frequent and important respiratory viral agent that causes respira-
tory infection complications in younger children and elderly. RSV
has an autumn/winter seasonality. Genetic diversity in both of RSV
A and B subtypes increased in last years with the spread of new
genotypes. This study aims to describe the genetic variability of
RSV during 2015/2016 season in Portugal and correlate the circu-
lating genotypes with detected ones in previous seasons. Will also
be evaluated the association between genotype, clinical diagnosis
and age.
Methods: During 2015/2016 winter season, between
November/2015 and February/2016, 45 RSV were genetically
characterized. RSV positive respiratory samples were collected in
two settings: children under 5 years old diagnosed by hospital
laboratories from the Portuguese Laboratory Network for the Diag-
nosis of Influenza Infection, and all age Influenza-like illness (ILI)
patients reported by primary care health services and diagnosed
by the National Influenza Reference Laboratory. All samples were
irreversibly anonymized. Demographic and clinical data were
collected. RSV detection was performed by real-time PCR and
other biomolecular methods. RSV genotype was assigned by the
nucleotide sequence of the hypervariable C-terminal region of the
G protein gene and the phylogenetic analysis was performed in
MEGA 6.0.
Results: From 45 RSV genetically characterized, 31 (69%) were
reported by hospitals, patients age ranged from newborn to 4 years
old. From these, 25 (81%; 25/31) patients were hospitalized, being
the bronchiolitis the most frequent diagnosis. While 14 (31%) RSV
cases came from primary care health services, patients age ranged
from 3 to 83 and all had a clinical diagnosis of ILI. Were included
patients from both genders in equal proportions.
RSV A and B co-circulated during 2015/2016 season. Were
genetically characterized 21 (47%) RSV A and 24 (53%) RSV B. 90%
(19/21) of RSV A clustered in ON1 genotype, the others 2 clustered
with NA1 genotype. All RSV B present a BA-like genotype, 70%
(17/24) were similar to BA9 and 30% (7/24) clustered with BA10
genotype.
Conclusions: During 2015/2016 season was observed a co-
circulation of RSVA and RSVB. In present study ON1genotype was
predominant in circulation among RSVA, this was also detected
as the major RSVA genotype at the global level. Only two RSVA
belonged to NA1 genotype. In Portugal, NA1 was in circulation dur-
ing 2010–2012 period. Undetected since 2012, it seems to reappear
during 2015/2016 season. All RSVB characterized belonged to BA
genotypes, themajority clusteredwithinBA9 genotype. BA10 geno-
type was also identified in circulation at low frequency. BA9 and
BA10 were being found in co-circulation since 2011/2012. No asso-
ciation was found between age, clinical diagnosis and RSVA and
B genotypes. RSV has an important impact in children in high-risk
groups highlighting the need of a continuous RSV surveillance each
winter.
Abstract no: 311
Presentation at ESCV 2016: Poster 205
Circulating enterovirus genotypes in Norway,
2014–2015: A reason for concern?
Natacha Milhano ∗, Kirsti Vainio ,
Andreas Christensen sanne Dudman
1 European Programme for Public Health
Microbiology Training (EUPHEM), European Centre
for Disease Prevention and Control (ECDC),
Stockholm, Sweden
2 Norwegian Institute of Public Health, Norway
3 St Olavs Hospital, Norway
Background: Enteroviruses (EV) are small single-stranded RNA
viruses belonging to the Picornaviridae family, and are responsible
for a wide variety of human infections ranging from mild respira-
tory illnesses to severe neurological diseases with central nervous
system involvement. In Norway, typing of enterovirus is performed
both at the Virology Department of the Norwegian Institute of
Public Health (NIPH) which is the National/WHO Reference Lab-
oratory for Polio/Enterovirus receiving samples from all regions of
the country and at St. Olav’s Hospital. Following an alarming detec-
tion of EV-D68 infections in young children presenting with acute
flaccid paralysis (AFP) in Norway in 2014, the aim of this study was
tomonitor the circulating EV genotypes obtained fromthe available
data collected from 2014 and 2015, in Norway.
Methods: We performed a retrospective descriptive study on
EV cases with clinical information dating from 2014 to 2015 by
analysing the distribution of isolates identified by serotyping using
neutralisation with monovalent antisera at NIPH; or identified by
Sanger sequencing at St. Olav’s Hospital. Statistical analysis was
performed using STATA software, with significance at p < 0.05.
Results: In general, the majority of EV positives was verified
among male (ranging from 52–56%) children below 2 years of age.
The analysis performed on NIPH data revealed that 23/132 (17.4%)
and 36/101 (35.5%) of samples were EV positive in 2014 and 2015,
respectively, of which 18/23 (78.3%) and 34/36 (94.4%) were typed.
The overall typing analysis for both years showed a predominance
of echo 18 and echo 30, with a threefold increase of Coxsackie B5
from 2014 to 2015. For St. Olav’s 2015 data, we verified that, from
a total of 1183 samples, 146 (12.3%) were EV positive, from which