Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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S124 Abstracts / Journal of Clinical Virology 82S (2016) S1–S142
Conclusion: HBoV1 DNA is frequently found in NPAs from chil-
dren with LRTI in Latvia. Although very often HBoV1 infection
is accompanied by co-infections with other respiratory viruses,
however there are LRTI cases when HBoV1 is the only pathogen
detected, indicating its possible role in etiology of the disease.
Abstract no: 316
Presentation at ESCV 2016: Poster 208
Molecular epidemiology of circulating human
coronaviruses in children at a tertiary hospital
in Catalonia (Spain) from 2014 to 2016
Javier Ramón ∗, Jorgina Vila, Cristina Andrés,
Cintia Castillo, Laura Gimferrer, María Pi˜nana,
María Gema Codina, Francisco Fuentes,
María del Carmen Martín, Rosario Saiz,
Pilar Alcubilla, Carlos Rodrigo, Tomàs Pumarola,
Andrés Antón
Hospital Universitari Vall d’Hebron, Vall d’Hebron
Research Institute, Universitat Autònoma de
Barcelona, Barcelona, Spain
Background: Human Coronaviruses (HCoVs) are single-
stranded, positive-sense RNA viruses. Four HCoVs species (229E,
OC43, NL63 andHKU1) are currently associatedwith asymptomatic
or mild upper-respiratory tract infections (URTI) in general popu-
lation, but severe acute respiratory infection (SARI) may occur in
patients with high risk of infection, such as immunocompromised
patients. The main aim of this study was to describe the seasona-
lity and genetic diversity of HCoVs, and the clinical features related
to HCoVs infection, in paediatric patients attended in our hospital
from 2014 to 2016.
Methods: FromOctober 2014 (week 40) to May 2016 (week 20)
respiratory specimens were collected frompaediatric patients who
were attended at the emergency care unit, outpatient departments
or admitted to Hospital Universitari Vall d’Hebron (Barcelona,
Spain) for diagnosis of respiratory viruses by Anyplex II RV16
Detection Kit (Seegene, Korea), that is only able to detect HCoV-
229E, HCoV-OC43 and HCoV-NL63, in addition to other respiratory
viruses. Partial RNA-dependent RNA polymerase gene (RdRp) was
sequenced from laboratory – confirmed HCoVs specimens for sub-
sequent phylogenetic analysis in order to confirm the routine
diagnostic PCR results. In addition, partial coding sequence of the
spike (S) glycoproteinwas sequenced to identify the different HCoV
genotypes. Clinical and epidemiological features of HCoV infected
cases were retrospectively reviewed from medical records.
Results: A total of 6661 specimens from 3900 patients were
received at our laboratory, of which 117 (2%) from96 patients were
positive for HCoVs (11 for HCoV-229E, 12%; 33 for HCoV-NL63, 34%
and 52 for HCoV-OC43, 54%). But, phylogenetic analysis of 61 par-
tial RdRp sequences revealed that viruses were belonging to the
four species (6 HCoV-229E, 9%; 15 HCoV-NL63, 25%; 22 HCoV-
OC43, 36%; and 18 HCoV-HKU1, 30%). HCoVs circulated throughout
the year, but highest number of detections were shown in autumn
months. Based on phylogenetic analysis of 69 S sequences: HCoV-
NL63 (32) fell into two clusters (16 A, 50%; 16 B, 50%); HCoV-OC43
sequences (19) in two clusters (5 B, 26%; 14 C, 74%); and HCoV-
HKU1 (18) mainly in other two (16 A, 90%; 1 B, 5%), but one (5%)
out of known genetic subgroups.
HCoV was more often found in respiratory samples of children
with URTI: 58% had URTI, of which 21% were associated with lower
respiratory tract infection (LRTI); 20.5% of patients had LRTI with-
out URTI; and, 21.5% were asymptomatic. HCoV-HKU1 (20%) and
HCoV-OC43 (29%) URTIs were less associated with LRTI than HCoV-
229E (50%) and HCoV-NL63 (40%). Most of children admitted with
HCoV LRTI required supplemental oxygen (11 out of 17 hospitalised
patients), but only 2 required it for more than 4 days. HCoV-229E
was related with more oxygen requirements, and HCoV-OC43 with
longer hospitalization stays. Only one case was admitted to Paedi-
atric Intensive Care Unit. No fatal cases due to HCoV infection were
reported.
Conclusions: Simultaneous circulation of the several HCoVs
species was shown from 2014 to 2016. Phylogenetic analysis
revealed the circulation of viruses belonging to different genetic
subgroups. Despite seasonal infection by these four HCoV species
is usually related to mild–respiratory disease, little differences in
the clinical features per specie were shown. Virological surveil-
lance must be done to detect changes on the virological and clinical
features related to circulating viruses.
Abstract no: 319
Presentation at ESCV 2016: Poster 209
No substantial circulation of enterovirus D68 in
patients with severe respiratory disease in
South-eastern Spain (Valencian Community)
during the 2015–2016 influenza season
Laura Cano ∗, Joan Puig-Barberà , Javier Díez ,
F. Xavier López-Labrador the Valencia
Hospital Network for the Study of Influenza and
Respiratory Viruses Diseas
1 Virology Laboratory, Genomics and Health Area,
Fundación para el Fomento de la Investigación
Sanitaria y Biomédica de la Comunitat Valenciana
(FISABIO)-Public Health, Valencia, Spain
2 Vaccines Research Area, Fundación para el Fomento
de la Investigación Sanitaria y Biomédica de la
Comunitat Valenciana (FISABIO)-Public Health,
Valencia, Spain
3 Centro de Salud Pública de Castellón, Castellón,
Spain
4 Consorcio de Investigación Biomédica de
Epidemiología y Salud Públic, Valencia, Spain
Background: Enterovirus-D68 (EV-D68) was associated with
severe respiratory disease in North America and other geographical
regions during the fall of 2014.
Methods: We compared the detection rates of EV-D68 in the
2014-2015 influenza season with that of the 2015-2016 sea-
son in samples collected in a prospective surveillance scheme
for all hospitalizations due to respiratory disease in our region
(Valencian Community, South-eastern Spain). Combined nasopha-
ryngeal and nasal (children <14 yr. old) or nasopharyngeal and
pharyngeal swabs are analyzed in a single laboratory at FISABIO-
Public Health for 16 respiratory viruses by multiplex real-time
RT-PCR, including rhinovirus/enterovirus as a single target. All
samples positive for rhinovirus/enterovirus were retested with a
rhinovirus/enterovirus discriminative real-time RT-PCR, and those
enterovirus positive for EV-D68 specific detection as a single target.
Results: In the 2014–2015 season, between November 15th and
March 31st, 372 of 4472 (8.32%) samples were rhino/enterovirus
positive, of which 66 (17.75%) were identified as enterovirus, and
15 (4.03%) confirmed as EV-D68. In the 2015–2016 season, between
November 15th and April 30th, 201 of 2700 (7.45%) samples were
rhino/enterovirus positive, of which 42 (20.82%) were identified as
enterovirus, and only one (0.50%) confirmed as EV-D68.