Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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Abstracts / Journal of Clinical Virology 82S (2016) S1–S142 S115
Abstract no: 221
Presentation at ESCV 2016: Poster 191
Microarray-based molecular detection of viral
pathogens associated with respiratory
infections
Muhammet Guzel Kurtoglu ∗, Oya Akkaya,
Ayse Ruveyda Ugur
Konya and Traditional and Research Hospital, Turkey
Objective: Acute respiratory infections are one of the most
common infectious diseases. Clinical course of acute respiratory
infections in young children and the elderly may be serious and
life-threatening. It has been emphasized that 20–60% of etiologic
agents are viruses. Respiratory syncytial virus (RSV) is the most
common viral pathogen in children. On the other hand, influenza
virus is the most commonly identified agent in adults. The aim of
this study is to identify the viral pathogens in hospitalized patients
with respiratory tract infection by using multiplex PCR method.
Methods: Nasopharyngeal swab samples obtained from hos-
pitalized patients with acute respiratory infections were enrolled
between January 2013 and December 2015. The identification of
influenza virus type A and B, human rhinovirus (HRV), respira-
tory syncytial virus A and B (RSV A-B), parainfluenza virus type
1, 2, 3, and 4, adenovirus, bocavirus, human coronavirus, human
enterovirus and human metapneumovirus (HMPV) in nasopha-
ryngeal samples was investigated by using CLART®Pneumovir kit
based on clinical array technology (Genomica, Spain).
Results: Of the 1290 patients included, 1110 (86%) were chil-
dren and 180 (14%) were adults. The number of samples in which
only one virus was identified was 600. In additional 150 specimens,
co-infections ofmultiple viruseswere detected. The total of positive
samples was 750 (58%). The majority of these positive specimens
were children’s samples (694 versus 56). RSV was the most com-
mon viral agent (35%) followed by HRV (13%), and influenza (10%).
The rate of co-existence of viral pathogens was 20%. The multiplex
pcr results were shown in
Conclusion: While RSV was the most common viral pathogen
detected inrespiratory infections, other emerging agents, such as
human metapneumovirus, bocavirus, and HRV were detected in
considerably high rates, suggesting these emerging agents should
not be underestimated in the etiology of respiratory infections. In
conclusion, in this study, it was shown that microarray-based mul-
tiplex PCR method is an easy, rapid, and sensitive diagnostic tool
for diagnosis of viral respiratory infections and that utility of this
methodmakes it essential among routine diagnostic tools in clinical
microbiology laboratories.
Table 1
Distribution of respiratory virusus in positive samples.
Viral agents % (n)
RSV 35 (266)
HRV 13 (94)
Influenza type A–B 10 (78)
Bocavirus 6 (45)
Parainfluenza virus 6 (44)
Metapneumovirus 5 (35)
Adenovirus 4 (30)
Enterovirus 0.7 (6)
Coronavirus 0.3 (2)
Co-existence 20 (150)
Total 100 (750)
Abstract no: 228
Presentation at ESCV 2016: Poster 192
Detection of influenza viruses from patients in
university hospital
Begüm Saran Gülcen ∗, Bahadır Feyzio˘glu ,
Mehmet Özdemir , Mahmut Baykan
1 Necmettin Erbakan University, Meram Medical
Faculty, Medical Microbiology Department, Medical
Virology Division, Turkey
2 Necmettin Erbakan University, Meram Medical
Faculty, Medical Microbiology Department, Turkey
Objective: Influenza epidemics occur almost every winter and
are associatedwith considerablemorbidity, mortality and econom-
ical lost. All age groups are susceptible, but increasing age, certain
chronic medical conditions, immundeficiency, pregnancy increase
the risk of complications and death. Influenzae A andB are responsi-
ble for annual epidemics. Quick diagnosis of influenza by laboratory
methods are important for treatment of flu and prevention of epi-
demics.
The aim of this study is to detect prevalence of influenza virus
and its subtypes in patients who admitted to University Hospital in
Konya province with flu like symptoms.
Material and methods: Nasal swap samples of patients with
flu like symptoms who admitted to various clinics in Necmet-
tin Erbakan University Meram Medical Faculty in Konya; Turkey
between January 1, 2013 and May 25, 2016 were tested for
Influenza A and Influenza B by CLART® PneumoVir (Genomica,
Madrid, Spain) micraarray method and Seeplex® RV12 ACE Detec-
tion multiplex PCR (Seegene, S. Korea).
Results: Results of total 2041 samples are analyzed retrospec-
tively. 258 (12.6%) samples were positive for influenza virus. 97
(4.7%) were found to be positive for influenza A and 161 (7.8%) for
influenza B.
Conclusion: Influenza B was found to be the predominant sub-
type in patients who admitted to hospital with flu-like symptoms
in Konya province. In our study January to March were the months
with the highest percentage of testing positive for influenza virus
infection. Influenza virus can be detected from respiratory sam-
ples with high sensitivity bymolecular methods such as microarray
method and multiplex PCR. Observing seasonal activity and epi-
demic strains and starting early treatment and taking isolation
measures are important for preventing rapid spread and progres-
sion of virus and has critical role for public health.
Abstract no: 233
Presentation at ESCV 2016: Poster 193
An overview of human parainfluenza virus 1-4
infections in northeastern Slovenia based on
molecular detection
Barbara Blaˇziˇc ∗, Darja Duh, Nika Volmajer,
Mojca Cimerman
National Laboratory of Health, Environment and
Food, Slovenia
Introduction: Human Parainfluenza viruses (HPIVs) are one of
the most common causes of upper and lower respiratory tract ill-
nesses, and an important cause of hospitalizations among children
under 5 years old. There are currently no published data on HPIV
infections in Slovenia.