Journal of Clinical Virology - Volume 82 - Supplement 1

S110

Abstracts / Journal of Clinical Virology 82S (2016) S1–S142

Laboratory tests and data analysis:

Poliovirus antibody detection

will be performed by neutralizing test. Specific antibody detec-

tion for the other agents is performed by immunoassay methods.

Chlamydia trachomatis

detection in urine will be carried out by real

time PCR.

Data from questionnaires and test results is entered into a

RedCap database by double-input. The statistical analysis will be

descriptive.

Expected results:

Determination of the prevalence for all infec-

tious agents enrolled in the current study, analysed by gender, age,

geographic distribution, and also regarding the socio-economic fac-

tors.

Expected outcomes:

The expected outcomes are to improve the

knowledge on the immunity profile of the Portuguese population

concerning VPD and provide population-based health information

to support the evaluation of the current National Vaccination Pro-

gram and also the implementation of national guidelines regarding

the prevention of sexually transmitted infections.

Acknowledgments:

This project is funded by Iceland, Liechten-

stein and Norway through the EEA Grants.

http://dx.doi.org/10.1016/j.jcv.2016.08.219

Abstract no: 275

Presentation at ESCV 2016: Poster 180

Mobile phones of paediatric senior physicians

are highly contaminated with viral genomes of

several epidemic viruses during the winter

season

A. Cantais

1 ,

∗

, A. Carlino

, F. Grattard

J. Gagnaire

, O. Mory

, B. Pozzetto

E. Botelho-Nevers

, S. Pillet

Paediatric Emergency Department, University

Hospital of Saint-Etienne, France

Laboratory of Infectious Agents and Hygiene,

University Hospital of Saint-Etienne, France

Hygiene Department, University Hospital of

Saint-Etienne, France

Infectious Diseases Department, University

Hospital of Saint-Étienne, France

Background:

Mobiles phones (MP) routinely used by health

care professionals are potential reservoirs of nosocomial bacteria,

but few data are available concerning viruses. A first study per-

formed in our centre (Pillet et al., Clin Microbiol Infect 2016) that

recorded the contamination by viral genome at a single day showed

that rotavirus RNA can be detected on MP, notably in paediatric

emergency and paediatric departments.

Objectives:

To correlate the circulation of epidemic viruses with

the contamination by RNA and DNA viruses on MP used by paedi-

atric senior physicians during all the winter season.

Study design

: Ten digital enhanced cordless telephones (DECTs)

were selected andwiped eachweek fromDecember 15 2015 toMay

3 2016 with a 480CE e-swab (Copan, Brescia, Italy). The swabs,

placed in transport medium, were frozen at

−

◦

C before viro-

logical analysis. A volume of 200 L of transport medium was

extracted by using the Specific B protocol on the NUCLISENS easy-

MAG instrument (bioMérieux, Marcy l’Etoile, France) under an

elution volume of 50 L. Respiratory viruses (respiratory syncytial

virus (RSV) A et B, influenza A et B, adenovirus, metapneumovirus,

coronavirus 229E, NL63 and OC43, parainfluenza virus 1, 2, 3 and 4,

bocavirus, enterovirus and rhinovirus) and gastro-intestinal viruses

[norovirus GI and GII, rotavirus A, adenovirus F (serotype 40/41),

astrovirus and sapovirus] were detected by RT-qPCR by using

Anyplex

II RV16 Detection kit and Allplex

Gastrointestinal Full

Panel Assay (Seegene, Eurobio, Courtaboeuf, France) respectively,

according to the manufacturer’s instruction. A questionnaire was

filled-in by the professionals before and after the study in order to

record their behavioural pattern in the use of MP and the hygienic

measures that they apply to these devices.

Results:

Among the 220 collected swabs, 110 (50%) were

positive for at least one viral genome. A total of 181 viruses

were identified. Multiples contaminations (up to 4 viruses)

were described on 53 swabs (48.2%). Adenovirus, bocavirus and

rotavirus, genomes were the most frequently detected on pro-

fessional MPs, with 71, 60 and 28 specimens found positive,

respectively. Adenovirus F, RSV-A, coronavirus OC43, coronavirus

NL63, rhinovirus and enterovirus genomes were less frequently

detected. The contamination of the MPs was correlated to the

viruses circulating the week before sampling. Half of the profes-

sionals declared to clean their phone at least once-a-week at the

beginning of the study and all increased the frequency of cleaning

at the end of the study.

Conclusions:

These results suggest that professional MPs used

by paediatricians are the vectors of a large panel of epidemic viruses

that circulated during the period study and may participate to the

dissemination of viral outbreaks in paediatric settings. As MPs are

not considered as medical devices, no formal recommendations are

currently available concerning hygienic measures related to their

disinfection. Promotion of MP cleaning together with hand hygiene

surrounding the use of MPs is urgently needed.

http://dx.doi.org/10.1016/j.jcv.2016.08.220

Abstract No: 280

Presentation at ESCV 2016: Poster 181

National serological survey – Portugal

2015–2016: Rubella seroprevalence in a

population-based sample of childbearing age

women resident in the North, Lisbon and

Algarve

P. Palminha

1 ,

∗

, S. Ferreira

, S. Moura

L. Almeida

1 , T. L

ourenc¸ o

1 , S. P

into

1 ,

R. Roquette

, B. Nunes

, C. Cardoso

, L. Brum

National Institute of Health, Dr. Ricardo Jorge,

Portugal

Clinical Laboratory, Dr. Joaquim Chaves, Portugal

Labco, laboratory network, Portugal

Background:

In Portugal, three different strategies for preven-

tion of congenital rubella have been implemented. The first started

in 1982 with the immunization of adolescent girls (aged 11–13

years) and non-immune women. The second began in 1987 with a

single dose of measles, mumps and rubella vaccine (MMR) for boys

and girls aged 15 months, followed by the introduction in 1990 of

the two MMR doses at 15 months and 11–13 years respectively.

Presently, rubella vaccine is routinely available asMMR; the first

dose is given at 12 months and the second at 4–6 years of age.

Aim:

Determination of rubella IgG seroprevalence in a repre-

sentative sample of women aged 20 to 44 years residents in three

territorial units (NUTII) of Portugal mainland.

Material and methods:

Study population

: The population-

based sample of women at childbearing age was collected in the

framework of the project National Serological Survey – Portugal

2015–2016 (project funded by Iceland, Liechtenstein and Norway

through the EEA Grants) and was calculated to be nationally repre-

sentative and corresponding to 42 women in the North and Lisbon