Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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Abstracts / Journal of Clinical Virology 82S (2016) S1–S142 S107
were subsequently used to understand their value in those
instances where no standard was available.
Conclusions: The WHO international standards contribute to
the standardisation of quantitative diagnostics. However, this study
shows that they are currently not commutable. In some cases, a
secondary standard is available for viral targets which still lack
a primary and WHO accepted international standard; agreement
between suppliers is low however. In the absence of international
standards for a large number of (viral) targets, new strategies can
and must be used to improve this lack of standardisation. We
theorise that when optimal diagnostic assays are used, a target-
independent universal standard can be implemented.
Abstract no: 326
Presentation at ESCV 2016: Poster 174
Half of family doctors do not use the flu vaccine
M. Altindis ∗, R. Karagoz , F.G. Aslan ,
M.B. Inci , A.R. Atasoy , S. Altindis
1 Sakarya University, Faculty of Medicine,
Department of Clinical Microbiology and Virology,
Sakarya, Turkey
2 Family Doctor, Turkey
3 Sakarya University, Faculty of Medicine,
Department of Public Health, Sakarya, Turkey
4 Sakarya University, Healthcare Management
School, Sakarya, Turkey
Questionnaires about Flu Vaccine on Primary Health Care Per-
sonnel Knowledge, Attitude andBehaviorwere administered to 180
Health Care Workers. 27.2% of respondents fever and headache,
arthralgia and 22.2% stated that the runny nose is the most obvi-
ous symptom of the flu. Flu a few times the last 3 years the rate
of 41.11% while the pass rate of only 23.88% who had once been
identified as influenza. Those who say I never pass rate is 13.33%.
The rate of influenza using the rest spend the last 3 years has been
seen as 77.22%. Participants in most patients with cough indoors
flu, with close contact with the patient and handshaking and kiss-
ing, stated that transmitted by close social contact. I place plenty
of fruits and vegetables to prevent the flu, herbal tea drink, as I
have stated Avoid contact with patients. “Did you make a flu vac-
cine in the past five years?” Is the question that is 49.44% of family
physicians ever make, the 27.22’s% and 23.33% for each year that
they stated that they only once. 63.33% of respondents think that
the only necessity of the vaccine in special cases, the 12.22% stated
he thought it was unnecessary. 30% of respondents stated that the
flu vaccine last 3 years even though the flu was built. The ratio
of non-believers that the vaccine is protective is determined as
35%. A participant recommended flu vaccine for health workers,
often those with the flu, the elderly, children, and with chronic ill-
ness. “Flu vaccine is given simultaneously with other vaccines” is
no question as to 71.11%, 13.88% gave the answer is yes.
Abstract no: 345
Presentation at ESCV 2016: Poster 175
Prevalence of specific IgGs against the study
Rubella Virus in Sao Tome and Principe with
“Guthrie Cards”
Vera Vicente ∗, Cristina Caroc¸ a ,
Maria Jesus Chasqueira , Paulo Paixão
1 CEDOC, Centro de Estudos de Doenc¸ as Crónicas,
NOVA Medical School/Faculdade de Ciências,
Portugal
2 Departamento de Otorrinolaringologia, NOVA
Medical School/Faculdade de Ciências Médicas,
Universidade Nova de Lisboa, Campo dos Mártires da
Pátria, 130, 1169-056 Lisboa, Portugal
3 Hospital CUF Infante Santo, Avenida Infante Santo,
34, 6◦ , 1350-079 Lisbo, Portugal
Introduction: Rubella it’s a contagious mild viral disease that
usually affects children. In pregnant woman, the fetal spread can
origin miscarriage or congenital rubella syndrome. São Tomé e
Príncipe is one of the countries where rubella vaccination is not
established. Some seroprevalence studies to other viruses have
used “Guthrie cards” (DBS), since they are easily collected, readily
stored and require minimum quantity of capillary blood.
Objective: Determination of rubella IgG seroprevalence in a São
Tomé e Príncipe population using DBS, validating previously this
method.
Material and methods: Population: DBS from 173 females and
143 males from São Tomé e Príncipe (ages between 2 and 35 years)
were collected between January and May of 2014.
- IgG extraction method: Elution of 32mm2 DBS (1 h/600 rpm,
followed by 16 h/4 ◦C).
IgG determination: SERION ELISA classic Rubella Virus IgG.
Method validation: Sensitivity and specificitywere evaluated using
the same ELISA technique in serum as reference.
Results: Method validation: Sensitivity: 89%; specificity: 100%.
Of the 316 analyzed samples we obtain 95 negative (<10UI),
203 positive (>15UI) and 18 borderline (10–15UI). The IgG’s preva-
lence against rubella virus in São Tomé e Príncipe was 65%. The
prevalence was similar throughout the island.
Conclusions: In our study DBS showed compatible results with
the referencemethod, suggesting that they canbe used for epidemi-
ologic surveillance in regionswith limited laboratory resources. The
high rubella seroprevalence noticed in São Tomé e Príncipe also
suggests that natural infections are very common in this area and
can be responsible for serious clinical consequences (congenital
rubella syndrome) in this population.