Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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S102 Abstracts / Journal of Clinical Virology 82S (2016) S1–S142
CMV resistance was detected in 20 patients (in 1, A594V was
detected before the HSCT). In two Ci-HHV-6 patients, ganciclovir
resistance (A594V and L595S) developed quickly after 39 and 53
days of treatment (126 and 186 days after HSCT) and so Ci-HHV-
6 positive recipient seems to be at higher risk of CMV resistance
development (p < .05).
Conclusions: As in our previous studies, we confirmed higher
frequency of Ci-HHV-6A in our cohort documenting presence and
quantity of HHV-6 DNA in the different biological materials and
tissues. Comparing to related CMV, HHV-6 was detected less fre-
quently. Impact of Ci-HHV-6 carriers on CMV infection seems to be
in Ci-HHV-6 positive recipient only among our patients.
Supported by grant of Internal Grant Agency of Ministry of
Health of Czech Republic NT/13691-4 and by the project for con-
ceptual development of research organization 00064203.
Abstract no: 348
Presentation at ESCV 2016: Poster 163
Monitoring of CMV infection: A comparison of
pp65-antigenemia from whole blood and Elisa
in Iranian patients undergoing kidney
transplantation
Mehdi Rahpeyma ∗ anochehr Makvandi
Alireza Samarbaf zadeh , Alireza gholami
1 Pasteur Institute of Iran, Iran
2 Ahvaz Jundishapur University of Medical Sciences,
Iran
Cytomegalovirus (CMV)-related disease is one of the most
frequent infectious complications after kidney transplantation
worldwide. Despite new therapeutic options, symptomatic CMV
infection still has a highmortality rate. In countries that CMV infec-
tion is common and more than 90% of general population is CMV
serology positive, PP65 antigenemia could be a good indicator of
recent CMV infection. In this study, the cytomegalovirus (CMV)
pp65 antigenemia assay was compared with detection of CMV IgG
in 50 kidney transplant patients. Antigenemia occurred only in
5 patients (10%) a median of 30 days (range, 14–74) after trans-
plant while all 50 kidney transplant recipients showed high level
of CMV IgG. one of 5 patients who presented with positive anti-
genemia developed fatal CMV pneumonia 10 days later. Thus, CMV
pp65 antigenemia may be useful in guiding antiviral treatment in
seropositive kidney transplant recipients.
Abstract no: 39
Presentation at ESCV 2016: Poster 164
Replication of porcine cytomegalovirus in
mesenchymal stem cells derived from
miniature pig bone marrow and lung
I.O. Ouh, J. Lee, J.E. Yu, H. Kang, I.S. Cho, S.H. Cha ∗
Viral Disease Division, Animal and Plant Quarantine
Agency, Gimcheon 39660, Republic of Korea
Introduction: Porcine cytomegalovirus (PCMV) belongs to the
genus cytomegalovirus, subfamily Betaherpesvirinae, family Her-
pesviridae, and is an icosahedral virus with a double-stranded
linear DNA genome. The virus particle diameter is 150–200 nm.
PCMV is distributed globally, with reported cases in Germany,
Japan, Britain and theUnite States. Serologic survey on this virus has
been reported in 76.3% of pigs but virological survey of PCMV distri-
bution has been reported in a fewpapers in Korea. Cytomegalovirus
have as a common feature the capacity for long-term virus per-
sistence after primary infection, resulting in latency. PCMV grows
slowly in cell cultures, and produces intranuclear inclusions in giant
cells. In this study, mesenchymal stem cells (MSCs) isolated from
miniature pig lung and bone marrow were infected with porcine
cytomegalovirus (PCMV).
Materials and methods: Korea PCMV 47-3 strain was isolated
fromperipheral bloodmononuclear cells (PBMCs) of a 8-weeks-old
pig without obvious clinical symptoms. Miniature bone marrow
derived mesenchymal stem cells (mp-BMSCs) and miniature pig
lung derived mesenchymal stem cells (mp-LMSCs) were infected
with Korea PCMV strain 47-3, porcine cytomegalovirus (ATCC VR-
1499) and then 16 days’ cultured. This culture supernatants sam-
pleswere screened by PCR amplification of DNA polymerase region.
Genomic DNA was extracted using DNeasy minikit (QIAGEN, USA).
The primer sets (sense: 5 -CCTATGTTGGCACTGATACTTGAC-3 ,
anti-sense: 5 -CCCTGAAAATCACCGTCTGAGAGA-3 ) were initially
used to amplify PCMV gene. Finally PCMV DNA was identified
by nested PCR (sense: 5 -ACGTGCAATGCGTTTTACGGCTTC-3 , anti-
sense: 5 -ACTTCTCTGACACGTATTCTCTAG-3 ).
Results: Cytopathic changes of mp-BMSCs and mp-LMSCs were
observed 16 days post infection. Cultures with CPE were further
analyzed for the presence of PCMV DNA by PCR. Different of mes-
enchymal stemcell was effectivemp-LMSCs better thanmp-BMSCs
in PCMV replication.
Conclusions: In this study, MSCs isolated from miniature pig
lung and bone marrow were infected with PCMV. PCMV isolation
was effective mp-LMSCs better than mp-BMSCs in PCMV replica-
tion. We also provided evidence that these cells are susceptible to
PCMV infection. Pigs are similar to human in anatomy, physiol-
ogy and immunological responses, and thus may serve as a useful
large animal preclinical model to study potential cellular therapy
for human diseases. Therefore, mp-LMSCs and mp-BMSCs should
be tested for PCMV before transplantation to prevent virus trans-
mission to recipients
Reference
[1] J.C. Booth, et al., Inclusion-body rhinitis of pigs: attempts to grow the causal
agent in tissue cultures, Res. Vet. Sci. 8 (1957) 338–345.
[2] J.F.L. Fryer, et al., Quantitation of porcine cytomegalovirus in pig tissues by PCR,
J. Clin. Microbiol. 39 (3) (2001) 1155–1156.
Abstract no: 72
Presentation at ESCV 2016: Poster 165
Monitoring of BK and JC polyomavirus viruria
and viremia in hematopoietic stem cell
transplant (HSCT) and renal transplant (RT)
recipients
M. Gozalo-Margüello ∗, I. Angulo-López ,
L. Martínez-Martínez , J. Agüero-Balbín
1 University Hospital Marqués de Valdecilla-IDIVAL,
Spain
2 University Hospital Marqués de Valdecilla-IDIVAL,
Department of Molecular Biology, University of
Cantabria, Spain
Background and objectives: Polyomaviruses are small, nonen-
veloped DNA viruses, which are widespread in nature. In
immunocompetent hosts, after primary infection the viruses