Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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S104 Abstracts / Journal of Clinical Virology 82S (2016) S1–S142
TTV viral load follow up: TTV was detected from 75% of donors
samples. Viral load ranged from 1.54 log to 5.59 in donors. In graft
recipients, the overall prevalence of TTV in bloodwas 95.2%. All TTV
positive patients raised their TTV load from an average of 5.34 log
to 8.89 in the first 75 days post transplantation.
Donors and recipient sample sequencing: the preliminary analy-
sis of the first sequencing chip (18 samples) showed the presence
of many TTV genotypes. On average, there are 2.1 million of
reads/sample.
Final sequencing results of all samples (genotypes and preva-
lence) will be presented with the goal to study the link between
detected TTV genotypes and others viruses. Biological and clinical
data will be correlated.
Conclusion: The high quality of the TTV R-gene® kit in terms
of analytical sensitivity, specificity, linearity and precision was
demonstrated. Due to a harmonized protocol and format, this kit
can be used in the same workflow that other R-gene® kits for trans-
planted patient monitoring potentially adding a tool tomonitor the
patients immune status. The analysis of TTV genotypes present in
kidneys of donors and recipients by sequencing adds useful infor-
mation to increase our knowledge about the correlation of TTV viral
loads and immune status of immunosuppressed patients in order
to improve individual treatment.
Abstract no: 79
Presentation at ESCV 2016: Poster 167
Rapid adenovirus typing method for species
identification
Fabienne Rayne ∗, Linda Wittkop ,
Clément Bader , Somar Kassab ,
Camille Tumiotto , Sylvie Berciaud ,
Harald Wodrich , Marie-Edith Lafon ,
Typadeno study members
1 UMR5234 Microbiologie fondamentale et
Pathogénicité, Université de Bordeaux, Bordeaux,
France
2 University Bordeaux, ISPED, F-33000 Bordeaux,
France
3 INSERM, Centre INSERM U1219, F-33000 Bordeaux,
France
4 CHU de Bordeaux, Pole de sante publique, F-33000
Bordeaux, France
5 Centre Hospitalier Universitaire de Bordeaux,
Bordeaux, France
Adenoviruses are characterized by a large variability, reflected
by their classification in species A to G. Certain species, e.g. A and
C, could be associated with increased clinical severity, especially
in immunocompromised hosts. Hexon sequencing represents the
most common method for Adenovirus typing. However, Adenovi-
rus capsid protein VI is characterized by significant interspecies
nucleotidic and gene size variability, which is conserved within a
given species and thus enables species differentiation.
Our group therefore designed a “pVI rapid typing method” to
obtain rapid and easy species assignment for Adenoviruses, thanks
to combined fusion temperature (Tm) and amplicon size analysis.
The method was established using plasmids encoding cloned pro-
tein VI genes from different Adenovirus species. In a second step,
the Typadeno study was started to compare the “pVI rapidmethod”
results to hexon Sanger sequencing results in 140 Adenovirus-
positive clinical samples.
In a subsample of 55 samples with results for both tests,
species A and C could be identified with a 100% positive predic-
tive value (95% confidence interval of respectively [39.76–100.00%]
and [83.89–100.00%]), thus confirming the potential value of this
simple typing method.
Our study is the first giving promising results for this new tech-
nique which would need to be confirmed in a larger assessment.
Abstract no: 12
Presentation at ESCV 2016: Poster 168
Genotyping and full genome sequencing of
varicella–zoster viruses isolated from Korean
patients
Min Ho Kim ∗ Kyo Kim an Hee Lee
Ho Sun Park
1 Department of Microbiology, Chungbuk National
University, Cheongju, South Korea
2 Department of Micorbiology, College of Medicine,
Yeungnam University, Daegu, South Korea
Varicella–zoster virus (VZV) is a causative agent for chickenpox
in primary infection and shingles after reactivation from latency.
Live attenuated vaccines have been developed based on Japanese
Oka strain and Korean MAV/06 strain. A number of complete or
near complete genomic DNA sequences have been determined for
genetic analyses. Recently, 3 clinical strains were isolated from
Korean patients and their genome sequences were completed
by high through-put sequencing technology. In this study it was
attempted to analyze the single nucleotide polymorphism (SNP) of
the VZV strains in order to understand the characteristics of Korean
clinical isolates. Phylogenetic analyses with 42 non-vaccine and
independent VZV strains including the 3 Korean strains YC01, YC02
and YC03 placed the 3 Korean strains to the clade 2 together with
pOka and LAX1. Comprehensive SNP analyses identified 87 sites
specific for each of the 5VZV clades. Clade 2 could be further divided
into 2 subclades: subclade 2a including pOka, LAX1 and YC01, and
subclade 2b including YC02 and YC03. Subclade 2a and 2b differed
at 6 SNP sites. The subclade 2b strains YC02 and YC03 also shared
similar bootscanning pattern distinct from the bootscanning pat-
tern of the subclade 2a strains. The subclade 2 strains appeared to
descend earlier than the subclade 2 strains from the most recent
common ancestor of the clade 2.
Abstract no: 121
Presentation at ESCV 2016: Poster 169
The distribution of enteroviruses isolated in
virology laboratory, Singapore General Hospital,
2008–2015
M.X.H. Tan∗, K.Y. Puong, K.P. Chan
Department of Pathology, Singapore General
Hospital, Singapore
Introduction: A study on the distribution of enteroviruses
was conducted to get a better understanding of the epidemiol-
ogy of enteroviruses in Singapore. The data were from 2008–2015,
when Singapore experienced several hand, foot and mouth dis-
ease (HFMD) epidemics caused by various viruses predominating
in different years.