Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

65 / 152

Abstracts / Journal of Clinical Virology 82S (2016) S1–S142 S61
years for norovirus, the 2014/2015 season showed the GII.4 (GII.4
Sydney 2012), remained the prevalent circulating strain.
Abstract no: 154
Presentation at ESCV 2016: Poster 80
Phylogenetic analysis of G12 group A rotavirus
circulating in Spain during 2012–2015:
Detection of different clusters with distinct
evolutionary origins
Cristina Santiso-Bellón ∗, Susana Vila-Vicent,
Jesús Rodríguez-Díaz, Javier Buesa
University of Valencia, Spain
Introduction: G12 groupA rotavirus (RVA) is currently regarded
as a common genotype in many geographical areas, in combina-
tion with several P-types. Rotavirus strains emerging in Europe in
the last 10 years include G8, G12 and G3P[8] genotypes. Analysis
of the different patterns of emergence for G12 strains has already
been published n Spain, G12P[8] RVA strains were described
for the first time in the Basque country in 2004–2005, being the pre-
dominant genotype during the 2010–2011 rotavirus season. Since
then, an increase of G12P[8] strains was detected in other Spanish
regions (Castilla-León, Aragón, Catalonia and Valencia), accounting
for 27.5% of all rotavirus-positive samples.
Objective: To analyse phylogenetically the G12 rotavirus strains
detected in different areas of Spain (Valencia, Zaragoza, Valladolid
and Barcelona) during the last seasons, in order to evaluate their
genetic variability and to compare their antigenic sites in VP7 and
in VP8* (VP4) with those of reference and vaccine strains.
Materials and methods: Viral dsRNA was extracted from 61
fecal samples using Trizol reagent. The G and P types were analysed
by RT-PCR following standardized procedures
). Nucleotide sequencing of VP7 (nt 88-876) and
VP4 (nt 88-876) amplicons was performed. Phylogenetic analyses
were performed using the MEGA6 software
). Trees were constructed using the maximum likelihood
method and the Tamura-3 parameter as a nucleotide substitution
model with the statistical support of 1000 bootstrap repetitions.
Results: Phylogenetic analyses of the VP7 and VP4 genes
demonstrated that they belong to lineages III of both G and P types.
These strains display the typical humanWa-like gene constellation,
and this may be the key to their recent increase and spread. Fifty-
fiveG12 stainswere associatedwith P[8] and sixwith P[6]. BothVP7
and VP4 (VP8*) genes could be separated into two different clusters,
with one of the VP4 (VP8*) clusters subdivided into two subgroups.
Antigenic regions were more conserved in VP7 than in VP4 (VP8*).
However, more diversity was found in the antigenic sites of VP7
when compared with the RVA vaccine strains. Our results suggest
different evolutionary origins for each genetic cluster.
Conclusions: The transmission and genetic evolution of
rotavirus strains depend on many factors, including biological fit-
ness, resistance to environmental stress and pre-existing immunity
in the population. In particular, VP7 and VP4 antigenic region vari-
ations may play an important role in the ability of RVA to escape
to the immune response. G12P[8] strains may have encounter all
these circumstances allowing their infectivity and rapid spread.
Reference
[1] Iturriza-Gómara, et al., Rotavirus genotypes co-circulating in Europe between
2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative
strain surveillance network, Epidemiol. Infect. 139 (2011) 895–909.
Abstract no: 16
Presentation at ESCV 2016: Poster 81
Detection of adenovirus in diarrheal children
between 0 and 5 years old and except
adenovirus serotype 40/41 by DNA sequencing,
and phylogenetic analysis
M. Colak ∗, G. Bozdayi , A. Altay , B. Dalgic ,
K. Ahmed
1 Gazi University Faculty of Medicine, Department of
Medical Microbiology, Ankara, Turkey
2 Gazi University Faculty of Medicine, Department of
Pediatric Gastroenterology, Ankara, Turkey
3 Department of Microbiology, Faculty of Medicine,
Oita University, Oita, Japan
Background:Adenovirus is one of themost important factors in
childrenwith acute gastroenteritis between the 0 and 5 years old all
over the world. In this study we aimed to determine the frequency
of adenovirus that is one of the viral gastroenteritis agents in chil-
dren between 0 and 5 years old and investigate the distribution
according to clinical findings, age groups, months and seasons.
Materials and methods: Stool samples were obtained from
180 children of 0–5 years old with acute gastroenteritis attended
between July 2007 and June 2011 to the Ankara Training and
Education Hospital. Stool samples were analysed by rapid chro-
matographic immune diagnostic test, enzyme immune assay (EIA)
and polymerase chain reaction (PCR). These methods that used in
the diagnosis of adenovirus were compared with each other and
discussed of the advantages of themethods. Sampleswere analysed
with hexon gene specific primers by PCR and DNA sequence analy-
sis and identified adenovirus types associated with gastroenteritis
outside 40/41. Phylogenetic analysis was made and adenoviruses
that have seen in our city were evaluated.
Results: The samples were found to be positive 5%(9/180)
by immune chromatographic method; 6.1%(11/180) by EIA;
13.9%(25/180) by PCR. Adenovirus gastroenteritis did not show any
difference in age group, gender, month and season. We identified
that vomiting in adenovirus gastroenteritis is an important finding,
but not common clinical table in the adenovirus gastroenteritis.
Most of the children that seen adenovirus gastroenteritis is with
diarrhoea and daily diarrhoea were found to be 6 and above were
observed. Compared to PCR, the sensitivity of the immune chro-
matographic method was 36% and specificity was 100%, PPV was
100% NPD was 90.6%; EIA test sensitivity has been identified as
44%, specificity 100%, PPV 100%, NPV 91.7%. In our study, 25 sam-
ples were found to be positive by PCR, 16(64%) for positive AdV41;
6(24%) for positive AdV40, 2(8%) for positive AdV31, 1(4%) for pos-
itive AdV7.
Conclusion: As a result of our work, it is shown that AdV31
and AdV7 can be associated with gastroenteritis with AdV40/41
serotypes. Highest frequency of adenovirus serotypes was 64%
with AdV41. In this study, genotyping and phylogenetic analy-
sis of enteric adenoviruses have been made for the first time in
our country. Adenovirus serotypes showed similarity with Asian
and American serotypes, 80% (20/25) and 20% (5/25) respectively.
Adenovirus serotypes that detected in our study were in concord-