Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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Abstracts / Journal of Clinical Virology 82S (2016) S1–S142 S57
Conclusion: This case is the first case of detection of Toscana
virus in the Southwestern France and demonstrates that this virus
could be a causative agent for aseptic meningitis in this area.
Furthermore our results showed that HSV1-2 and HHV-6 are
very rarely the causative agents of meningitis among patients older
than five year-old.
Abstract no: 268
Presentation at ESCV 2016: Poster 72
From TYPENED to REGIOtype to EUROtype:
Moving towards a comprehensive surveillance
strategy for emerging viruses
R. Poelman ∗, D. Luijt , R. van Rhee-Luderer ,
W. Niessen Rahamat-Langendoen
M.K. van Genne , C.C. Van Leer-Buter ,
H.G.M. Niesters
1 University Medical Center Groningen, University of
Groningen, Department of Medical Microbiology,
Division of Clinical Virology, Groningen, The
Netherlands
2 Certe, Department of Medical Microbiology,
Groningen, The Netherlands
3 Izore, Center for Infectious Diseases Friesland,
Leeuwarden, The Netherlands
4 Regional Public Health Service (GGD), Department
of Infectious Disease Control, Groningen, The
Netherlands
5 Radboud Univeristy Medical Center, Department of
Medical Microbiology, Nijmegen, The Netherlands
Background: Patients with respiratory illness are screened for
viral infections. This screeningwas expanded by routinely sequenc-
ing enteroviruses and rhinoviruses to identify outbreaks, new
viral strains and transmission patterns. Additionally, we included
noroviruses and parechoviruses in patients with gastro-intestinal
complaints or signs of encephalitis/meningitis. For surveillance
purposes, a national structure called TYPENED is available through
the Dutch National Institute of Health (RIVM). Our main objective is
to provide a rapid regional sequencing service that create a source
of clinical information and to correlate this with epidemiological
patterns.
Methods: A sequencing and epidemiological strategy for
surveillance, called REGIOtype, was implemented by including
GP’s, regional hospitals and regional Public Health Services which
covers the Northern part of the Netherlands, for a rapid flow of
informationwithin our region. Sequencing is performed locally and
results are available within one week. Clinical data are exchanged
and phylogenetic analysis is performed.
Results: Phylogenetic analysis has contributed to the knowl-
edge of circulating viral strains and the relatedness within
and between multiple institutions. In total 2629 samples were
processed for sequencing within a 2 years period. 2223 of these
samples (85%) were successfully sequenced, the other 406 (15%)
failed, mainly due to a low viral load. 443/2223 (20%) were
enterovirus, 891 (40%) rhinovirus, 768 (35%) norovirus and 121 (5%)
parechovirus. In total, 962/2223 (43%) sequences were obtained
from healthcare institutions in the region and 1261 (57%) from the
university hospital. Enterovirus D68 and recently the ‘new’ C-group
enteroviruseswere identifiedusing this strategy aswell as a vaccine
derived poliovirus strain from a refugee.
This rapid sequencing strategy in combination with the clini-
cal data enables the application of infection control measures in
the participating (local) health institutions. Besides, these data not
only indicated a possible outbreak, but it also improved detection
methodologies in our regional laboratories.
Conclusion: REGIOtype has proven to be an adequate strat-
egy for surveillance on local circulating viruses and not only for
the hospitalized populationwith severe (underlying) illnesses. This
underlines the importance for regional surveillance by sequenc-
ing, of which several networks are available within Europe but still
acting independently from each other.
Abstract no: 294
Presentation at ESCV 2016: Poster 73
Multicenter investigation of bufavirus in the
etiology of viral central nervous system
infections of adults and children
Aylin Altay ∗, Murat Ocal ,
Saliha Kanik Yuksek , Meltem Polat ,
Anil Tapisiz , Hasan Tezer , Koray Ergunay ,
Gulendam Bozdayi , Kamruddin Ahmed
1 Department of Medical Microbiology, Gazi
University, Ankara, Turkey
2 Department of Medical Microbiology, Hacettepe
University, Ankara, Turkey
3 Ministry of Health, Diskapi Dr. Sami Ulus Training
and Education Hospital, Ankara, Turkey
4 Department of Paediatrics, Faculty of Medicine,
Gazi University, Ankara, Turkey
5 Department of Pathobiology and Medical
Diagnostics, Faculty of Medicine and Health Sciences,
University Malaysia Sabah, Kota Kinabalu, Malaysia
6 Department of Microbiology, Faculty of Medicine,
Oita University, Yufu, Japan
7 Research Promotion Institute, Oita University,
Japan
Introduction: Bufavirus (BuV) is a newly-identified parvovirus,
detected in the diarrhoeal stool samples of patients from Burkina
Faso. The global distribution, epidemiology and genetic characteris-
tics of BuVs infections are obscure. Considered primarily as an agent
causing gastroenteritis, the association and outcome of BuV infec-
tions with various clinical presentations mostly remain explored.
This study was undertaken to investigate probable impact of BuV
in central nervous system infections in a region where it was pre-
viously reported to cause human infections
Methods: The study was undertaken in 3 institutions in Ankara
province, Central Anatolia, Turkey. Patients, clinically diagnosed
with febrile disease and/or central nervous system infections of
presumed viral aetiology, were enrolled in the study with informed
consent. Sera and/or cerebrospinal fluid specimens were collected
from 95 children attended to Gazi University Hospital and Diskapi
Training and Education Hospital from during 2011–2013 and 38
adult patients, attended to Hacettepe University Hospital from
June 2012 to March 2013. Clinical history and follow-up, physical
examination and standard laboratory findings of the patients were
recorded. Nucleic acid extraction was performed via commercially
available spin-column assays and BuV detection was carried out by
in house nested-PCR utilising previously-described primers.
Results and conclusion: In all patients, bacterial, mycobacte-
rial and fungal cultures were negative, as well as PCR for herpes
simplex virus (HSV) types 1/2. PCR results of all samples were
negative for BuV. This is the first study that evaluates a probable
association of BuV and central nervous system infections. Although