Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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S64 Abstracts / Journal of Clinical Virology 82S (2016) S1–S142
real-time RT-PCR detected 39% (82/210) RV-positive samples. NoV
GI and GII were detected by conventional RT-PCR in 11.6% (21/181)
and in 24.8% (45/181) samples, respectively. The analysis of the
same specimens by the VIASURE real-time assays yielded 11.6%
(21/181) NoV GI-positive and 27% (49/181) NoV GII-positive sam-
ples, whereas the RIDAGENE tests yielded 13.2% (24/181) NoV
GI-positive and 27% (49/181) NoVGII-positive results. The VIASURE
Norovirus GI and GII assays detected the following NoV genotypes:
GI.1, GI.2, GI.4, GI.9; GII.1, GII.4 (five variants), GII.7, GII.17, and
GII.21.
Conclusions: The sensitivity of the VIASURE Rotavirus Real Time
PCR assay increases 1.23 times those of the ICG assay and the
end-point RT-PCR. Rotavirus was detected by both evaluated real-
time assays with a total agreement rate of 94.8%. For norovirus GI
and GII detection, the total agreement rate between VIASURE and
RIDAGENE real-time assays was 96.1% and 98.9%, respectively. The
specificity of the assays is pending on the sequence analysis of the
mismatched positive samples.
Abstract no: 25
Presentation at ESCV 2016: Poster 86
Epidemiology of Astrovirus infection in infants
in Tehran, Iran
D. Behzadpour ∗, S.D. Musavinasab
1 Islamic Azad University, Rasht Branch, Rasht, Iran
2 Department of Virology, Faculty of Medical
Sciences, Tarbiat Modares University, Iran
Background: Human astroviruses (HAstVs) are positive-sense
single-stranded RNA viruses that were no enveloped particles with
a distinctive star-shaped surface structure that have been were
discovered in 1975 by electron microscopy in stool samples
HumanHAstVs classified into the generaMamastrovirus and Astro-
virus and have emerged as another common cause of non-bacterial
acute gastroenteritis Limited data exist on the epidemiology
and genetic diversity of HAstVs in Iran. This study assessed the role
of human Astrovirus (HAstV) at the 120 gastroenteritis samples to
determine the prevalence, time distribution, and medical signifi-
cance of human Astrovirus infection among children 5 years old in
Tehran, Iran.
Materials and methods: Stool samples were collected from
Children’s Medical Centers in Tehran, Iran from May 2013 to May
2014. HAstV was detected using RT-PCR and positive samples were
subsequently tested for other common viral as well as Rotavirus
and Norovirus
Results: The overall incidence of Astrovirus was found to
be 6.6%. Mixed infections with other viral enteric agents were
detected in 2.5% of all Astrovirus-positive samples. During the 1-
year period, the highest Astrovirus incidence was reported in the
winter months, although infections also occurred in spring.
Conclusion: Fromour data for each age group, we observed that
HAstV affected mostly children younger than 1 years of age, while
HAstV had a greater impact inMale children. Our study provides an
epidemiological overviewof HAstVs associatedwith acute diarrhea
in Iran.
Reference
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Abstract no: 29
Presentation at ESCV 2016: Poster 87
Surveillance of Noroviruses GII.4 variants, GII.3
and GII.17 in acute gastroenteritis patients in
Shenzhen, China from 2012 to 2015
Yaqing He ∗, Yaqing He, Ning Han, Hailong Zhang,
Long Chen, Yujie Wang, Kaifeng Wang,
Baihua Shu
Shenzhen Center for Disease Control and Prevention,
China
Background: Norovirus (NoV) is a leading cause of acute gas-
troenteritis (AGE) in individuals of all ages. Shenzhen, an immigrant
metropolis connecting mainland China and Hong Kong, has a high
population density and mobility, which make Shenzhen an AGE-
prone area allowing for centralized surveillance of NoV-associated
activities. The goals of this study were to determine the etiology
of NoV-associated AGE and to figure out the epidemiological char-
acteristics of NoV epidemic strains (GII.4 Den Haag 2006b, GII.4
Sydney 2012, GII.3 and GII.17) in Shenzhen, China.
Methods and specimens: From 2012 to 2015, a total of 4432
stool specimens were collectedwith AGE patients’ data. Therewere
18 different NoV serotypes identified by real time RT-PCR and one
step RT-PCR with N/S primers GI-SKF/GI-SKR and COG 2F/G2-SKR,
including GII.4 Den Haag 2006b, GII.4 Sydney 2012, GII.4 can not
assign, GII.2, GII.3, GII.6, GII.7, GII.12, GII.13, GII.17, GII.21, GI.1-GI.6
and GI.8. Results, from 2012 to 2015, GII.4 Sydney 2012 accounted
for the majority proportion of AGE (29.41–75.83%), followed by
GII.3 (2.61%-10.00%). GII.4 Den Haag 2006b (49.57%) was the pre-
dominant serotype in 2012. As the novel pathogen in Shenzhen,
GII.17 (10.43–21.74%) became as a common causative agent of AGE
in the winter season of 2014–2015. Statistical analysis indicated
that the median (QR) age of AGE patients infected with GII.4 Syd-
ney 2012 (21.50 [31.75]) was the highest compared to GII.4 Den
Haag 2006b (1 [3.83]) and GII.3 (0.58 [0.58]) (P < 0.001) in 2012; the
median (QR) age (1.00 [32.25]) of GII.4 Sydney 2012-infected AGE
patients was higher than that (0.58 [2.17]) of GII.3-infected AGE
patients in 2013 ( P< 0.05); the median (QR) age (30.00 [26.25]) of
GII.17-infected AGE patients was the highest compared to that of
GII.4 Sydney 2012 (1 [12.33]) and GII.3 (0.67 [10.58]) (P < 0.001) in
2014, which was similar to that in 2015. The male/female ratio was
different among these four prevalent NoV serotypes in 2012, 2014
and 2015. Phylogenetic analysis based on N/S gene revealed that
NoV strains in Shenzhen were closely homological to that in the
neighbour cities and countries.
Conclusion: The diversity of NoV has been surveilled that GII.4
variants, GII.3 and GII.17 were the prevalent strains in Shenzhen,
China, from 2012 to 2015. Continued monitoring and comprehen-
sive analyses of NoV is necessary for a better understanding of their
evolution, epidemiology, prevention and control measures.
Keywords:Norovirus, Acute gastroenteritis, GII.4 variants, GII.3,
GII.17.