Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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Abstracts / Journal of Clinical Virology 82S (2016) S1–S142 S51
ples from blood bank donors in the United States (measles n = 399,
mumps n = 399, and VZV n = 399) were analyzed with the BioPlex
2200 MMV IgM assay. The results were compared to the DiaSorin
Liaison measles IgM, mumps IgM and VZV IgM assays. The BioPlex
MMV IgM assay was further evaluated for imprecision around the
cutoff. Time to first result and throughput were also observed.
Results: Among the presumptive positive sample population,
the BioPlex MMV IgM assay showed a positive agreement of 96.1%,
92.3%, and 95.2% for measles, mumps and VZV IgM, when com-
pared to the Liaison Measles IgM, Mumps IgM and VZV IgM assays.
The test ordered sample population had a positive prevalence of
1.0%, 5.0%, and 2.0% for measles, mumps, and VZV IgM respectively.
Among the sample set taken from a healthy population the MMV
IgMassay showed a negative agreement of 99.5%, 99.2%, and 100.0%
for measles, mumps and VZV IgM respectively. The imprecision
for positive samples was shown to be between 4.1 and 8.5% for
measles IgM, 4.4–10.7% for mumps IgM, and 3.4–8.2% for VZV IgM.
The standard deviation of results from the negative samples was
<0.05 SD across all three analytes. The MMV IgM assay has a time
to first result of 45min, throughput of 63 samples per hour, and
offers up to 189 results per hour by utilizing multiplex technology.
Conclusions: The data demonstrate the BioPlex 2200 MMV IgM
assay is comparable to commercially available assays and that this
new assay meets laboratory needs for precision and throughput.
By combining the three analytes together in a single test reaction,
the BioPlex 2200 MMV IgM assay provides a complete and more
efficientmethod to assist in the diagnosis of these highly contagious
diseases.
Abstract no: 80
Presentation at ESCV 2016: Poster 60
Paper-based point-of-care testing for
cost-effective diagnosis of acute dengue
infections
F. Bedin ∗, E. Voilin, L. Boulet, G. Theillet, A. Perrin,
C. Rozand
bioMerieux SA, France
Dengue is a serious healthcare concern in tropical and sub-
tropical countries. Although well-established laboratory tests can
provide early diagnosis of acute dengue infections, access to these
tests is limited in developing countries, presenting an urgent need
to develop simple, rapid, and robust diagnostic tools. Paper-based
point-of-care (POC) devices, are typically rapid, cost-effective and
user-friendly, and they can be used as diagnostic tools for the
prompt diagnosis of dengue at POC settings. The early and prompt
diagnosis is crucial to improve patient management and reduce the
risk of severe dengue complications.
Here we have developed and evaluated a wax-printed paper-
based device for the detection of the non-structural NS1 dengue
viral protein in blood and plasma. The requirements to obtain both
the highest specific signal and the lowest background have been
studied. The rawmaterials quality and the washing steps have been
determined as crucial. With the developed method we were able
to detect specifically in 5–6min less than 10 ng/mL of protein in
various sample types. The read of the resultswas simplified by using
a dedicated application on a smartphone.
Abstract no: 86
Presentation at ESCV 2016: Poster 61
Loop-mediated isothermal amplification for
point-of-care diagnosis of viral respiratory tract
infection in childhood
R. Peters ∗, J. Pfeil , J. Tabatabai ,
J. Grulich-Henn , P. Schnitzler
1 Department of Infectious Diseases, Heidelberg,
Germany
2 Centre for Child and Adolescent Medicine,
Heidelberg, Germany
Background: Acute respiratory tract infection (ARTI) caused by
respiratory syncytial virus (RSV) is a frequent cause of hospital-
ization in young children. The prevention of nosocomial infection
therefore is crucial to reduce morbidity and mortality in severely
ill patients.
Objective: The aim of this study is to apply novel isothermal
amplification methods for point-of-care testing in children hospi-
talized with ARTI.
Methods: We evaluated a novel DNA amplification technique,
called “Loop-mediated Isothermal Amplification” (L-AMP) allow-
ing highly sensitive detection of RSV frompurified viral RNAwithin
approximately 30min at comparably low costs. To evaluate the test
results we compared them with real-time PCR as a gold standard.
Nasopharyngeal swabs (mSwab, Copan, Italy) and clinical datawere
obtained from hospitalized children who presented with symp-
toms of ARTI.
Results: The L-AMP test was first evaluated using patient sam-
ples in a laboratory setting during the winter season 2014/2015
and showed a test sensitivity of 73% compared to 58% sensitivity
of the conventional RSV rapid antigen detection test. In winter sea-
son 2015/16, we transferred the technology to a routine application
in a point-of-care setting at the Paediatric Department of the Uni-
versity Hospital Heidelberg. From November 2015 until April 2016
336 swabs were collected, of which L-AMP results were obtained
for 326 samples. Mean age of all children was 21.9 months and
43.5% were female.
In total 108 swabs (32.1%) were RSV-positive. The L-AMP test
showed a sensitivity of 70.2% and a specificity of 96.8%.
Conclusion: This innovative approach could substantially
enhance the accuracy to detect ARTIs in a point-of-care setting.