Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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Abstracts / Journal of Clinical Virology 82S (2016) S1–S142 S85
Abstract no: 93
Presentation at ESCV 2016: Poster 129
Detection of Q80K mutation in HCV NS3
protease gene in Hradec Kralove – Initial
experience
Lenka Pliskova ∗, Radka Kutova ,
Stanislav Plisek , Vlasta Stepanova
1 Inst. Clin. Biochemistry and Diagnostics –
Department Mol. Biol., University Hospital and
Faculty of Medicine, Charles University, Hradec
Kralove, Czech Republic
2 Clin. Biochemistry and Diagnostics – Department
Mol. Biol. University Hospital and Faculty of
Medicine, Charles University, Hradec Kralove, Czech
Republic
3 Clinic of Infectious Diseases, University Hospital
and Faculty of Medicine, Charles University, Hradec
Kralove, Czech Republic
4 Inst. Clin. Microbiology – Virology Department,
University Hospital and Faculty of Medicine, Charles
University, Hradec Kralove, Czech Republic
Background: Combination of HCV high speed replication, low
accuracy and poor HCV polymerase correction result in formation
of highly variable viruses overall called “quasispecies” with a high
sequential diversity within different genotypes and subtypes. The
impact of mentioned situation is the accumulation of virus vari-
ants with mutations (originated in aminoacids substitution) with
the different grade of resistance to DAA (directly acting antivirals)
in naive patients, so called RAV (resistance associated amino acid
variants)
PolymorphismQ80K in NS3 region belonging also to RAV occurs
most often in patients infected with HCV 1a genotype and is asso-
ciated with a decreased response to simeprevir therapy. Q80K
prevalence in these patients varies in different geographical con-
ditions. The study of Q80K prevalence in European population
presents the prevalence of 19.8% in patients with genotype 1a and
0.5% in genotype 1b %
At present the patients infected with HCV genotype 1a are
examined for the presence of mutation in Q80K codon prior
to simeprevir therapy introduction. Further clinically significant
mutations described in codons 36, 43, 122, 138, 155, 156, 158, 168
are reported only in 1–2% of cases
Materials and methods: Our laboratory of molecular biology
introduced the detection of mutations in the gene for protease NS3
in 2015 bymeans of sequence analysis. The primers designwas per-
formed using Custom Primers – OligoPerfectTM Designer software.
The method was optimized for HCV genotype 1a.
Results: In the period of September 2015 to May 2016 the total
of 45 patients were examined, in 10 of them (22%) Q80K mutation
was detected.
Reference
[1] M. Ogishi, H. Yotsuyanagi, T. Tsutsumi, et al., Deconvoluting the composition of
low-frequency hepatitis c viral quasispecies: comparison of genotypes and NS3
resistance-associated variants between HCV/HIV coinfected hemophiliacs and
HCV monoinfected patients in Japan, PLOS ONE 10 (2015) 1–28.
[2] S. Paolucci, L. Fiorina, B. Mariani, et al., Naturally occurring resistance
mutations to inhibitors of HCV NS5A region and NS5B polymerase in DAA
treatment-naïve patients, Virol. J. 10 (2013) 355–361.
[3] M. Leggewie, V.B. Greenu, T. Abdelrahman, et al., Natural NS3 resistance
polymorphisms occur frequently prior to treatment in HIV-positive patients
with acute hepatitis C, AIDS 27 (2013) 2485–2488.
[4] C. Sarrazin, E. Lathouwers, M. Peeters, et al., Prevalence of the hepatitis C virus
NS3 polymorphism Q80K in genotype 1 patients in the European region,
Antivir. Res. 116 (2015) 10–16.
[5] A. Bae, S.C. Sun, X. Qi, et al., Susceptibility of treatment-naive hepatitis C virus
(HCV) clinical isolates to HCV protease inhibitors, Antimicrob. Agents
Chemother. 54 (2010) 5288–5297.
Abstract no: 99
Presentation at ESCV 2016: Poster 130
Hepatitis B virus vaccination status of medical
laboratory workers; a multicentre evaluation in
Turkey
O. Aydemir , M. Koroglu , B. Yuksel ,
T. Demiray , A. Ozbek , S. Altindis , F.G. Aslan ,
M. Altindis BIOSAFETYGROUPT
1 Sakarya Research and Training Hospital
Microbiology Lab, Sakarya, Turkey
2 Sakarya University Faculty of Medicine Dept of
Clin. Microbiology, Sakarya, Turkey
3 Sakarya University Faculty of Management, Health
Administration, Sakarya, Turkey
4 Sakarya University Faculty of Medicine Dept of
Clin. Virology and Microbiology, Sakarya, Turkey
5 Keramettin Yanik (On Dokuz Mayıs University
Faculty of Medicine, Department of Medical
Microbiology, Samsun), Sebahat Aksaray
(Haydarpas¸ a Research and Education Hospital,
Microbiology Laboratory, ˙Istanbul), Nevzat Unal,
Turkey
Introduction and aim: The frequency of hepatitis B infection
among health care workers is reported to be 3–8 times more than
the normal population, particularly among workers in emergency
service, surgery, intensive care unit and laboratory, who are fre-
quently exposed to the contaminated patient materials such as
blood and other body fluids n this multicentre study, we aimed
to determine the rates of hepatitis B vaccination in medical labo-
ratory workers in Turkey and aimed evaluate the precautions to be
taken on this special subject.
Materials and methods: Total number of 1359 medical labora-
tory workers from 28 medical centres representative of different
regions of Turkey was included in this study. A questionnaire was
designed to gather all the data on the subject planned to apply all
the medical laboratory workers.
Results: Total number of 1359 laboratory worker was included
in this study, and male to female ratio was 0.74 (578/781). Doc-
tors (n = 133), research assistants (n = 78), laboratory technicians
(n = 196), biologists (n = 750), students (n = 24), cleaning staff and
other workers (n = 161) were included in the study. We deter-
mined that HBVvaccinewas applied to the 1118 laboratoryworkers
(82.3%) out of 1359. When anti-HBs titre levels of the vaccinated
participants were investigated, 715 (54.5%) of the vaccinated par-
ticipants stated that they had anti-HBS levels above 10 IU/mL, 116
(8.5%) of them told that their antibody levels were below 10 IU/mL
and 502 (36.9%) of them stated that they did not know their anti-
HBS titre levels. The results of statistical analysis revealed that
vaccination rates and occupation groups were correlated among
the laboratory staff (p < 0.05). However, there was no significant
difference between age groups and the duration in work with the
vaccination rate (p > 0.05). Anti HBs positivity was not correlated
with any of the groups (p > 0.05).
Discussion and conclusion: Health care professionals are
required to make immunization a professional habit to protect
themselves from health care associated infections in addition to
implantation of standard infection control procedures resent