Journal of Clinical Virology - Volume 82 - Supplement 1 - September 2016

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Abstracts / Journal of Clinical Virology 82S (2016) S1–S142 S135
Slovakia and there are no data available on the incidence or the
long-term outcomes of congenital CMV infections.
For a period of eight years (from April 2008 to April 2016),
we retrospectively determined the number of amniotic fluids and
dried blood spots (DBSs) analysed for the presence of CMV DNA
in our laboratory, as well as the number of cCMV symptomatic
neonates born during this time period. In total, 58 amniotic flu-
ids, 23 DBSs and samples from 182 newborns (0–14 days old) with
clinical symptomswere analyzed for the presence/viral load of CMV
DNA by nested end-point PCR and/or quantitative real-time PCR.
CMV DNA was detected in 3 amniotic fluids (2.5 ×10 3; 1.6 ×105
and 3.9 ×10 7 IU/ml, respectively), 7 DBSs, and in samples from 18
neonates (whole blood, urine or both).
In the period under review; 84,909 women were examined
for syphilis as a part of compulsory screening for infectious dis-
eases in pregnancy. The relatively low number of investigations
for cCMV infections demonstrates the lack of awareness about the
risks associated with primary and/or non-primary CMV infections
in pregnant women.
Abstract no: 102
Presentation at ESCV 2016: Poster 232
Study of enterovirus and parechovirus
infections in young children in Spain over a
3-year period
María Cabrerizo ∗, F. Martín Del Valle ,
C. Mu˜noz-Almagro aunes P. Romero
A. Moreno-Docón , A.I. Menasalvas ,
L. Reis-Iglesias , J. García-Costa , A. Cilla ,
G. Megias , S. Rey-Cao , M. Mar Portugués ,
M.J. Pena , M. Del Cuerpo , N. Rabella ,
M. Aranzamendi , A. Martínez-Sapi˜na ,
S. Sanbonmatsu , A. Otero , C. Calvo
1 National Centre for Microbiology, Health Institute
“Carlos III”, Madrid, Spain
2 Hospital Severo Ochoa, Leganés, Madrid, Spain
3 Hospital San Joan de Deu, Barcelona, Spain
4 Hospital La Paz, Madrid, Spain
5 Hospital Virgen de la Arrixaca, Murcia, Spain
6 Complejo Hospitalario de Orense, Spain
7 Complejo Hospitalario de Burgos, Spain
8 Hospital Meixoeiro, Vigo, Pontevedra, Spain
9 Hospital Gran Canaria, Las Palmas de Gran
Canarias, Spain
10 Hospital Santa Creu i Sant Pau, Barcelona, Spain
11 Hospital Cruces, Bilbao, Spain
12 Hospital Miguel Servet, Zaragoza, Spain
13 Hospital Virgen de las Nieves, Granada, Spain
Introduction: Human enteroviruses (EVs) and more recently
parechoviruses (HPeVs) have been recognized as important viral
causes of neurological and systemic infections in children. Our aim
was to investigate the epidemiology of EV and HPeV infections
and their clinical association in young children over a 3-year study
period in Spain.
Patients and methods: Prospective and multicentre study
(Grant AES PI12-00904) performed in children <3 years of age
admitted in 12 Spanish hospitals during 2013-2015. EV and HPeV
infections were investigated in cerebrospinal fluids, sera or throat
swabs from patients with fever without source (FWS), suspicion
of clinical sepsis, meningitis or encephalitis. Clinical data and
informed consent were recorded. EV and HPeV detection and geno-
typing in clinical samples was performed by RT-PCR and further
sequencing.
Results: A total of 786 patients were included in the study. The
mean age of the children was 5.2 + 9.1 months and 46% of them
were neonates (<1 month). Male/female rate was 1.5. Of the 786
samples analysed, 420 (53%) were EV-positive and 45 (6%) were
HPeV-positive. 27 different EV types (5 EV species A and 22 EV
species B) were identified while all but 3 HPeV were type 3. Overall
the 4 types detected most frequently were echovirus (E)-16 (10%),
HPeV-3 (9%), E-6 (8%) and E-18 (6%). All HPeV-infected children
were <2 months, being their mean age significantly lower than in
EV-infected patients (1.7 + 4.9 vs. 5.9 + 10.1 months, p = 0.008). The
highest incidence of EV infections was between April and July each
year, with another small peak in autumn. HPeV-3 circulation was
also higher in spring and early summer, but it seems to be bian-
nual. Clinically, EV infections were associated with meningitis (29%
vs. 0%, p = 0.001), pleocytosis (40% vs. 4%, p = 0.0006) and higher
leucocytes (10200 + 5000 vs. 7200 + 3800 cells/mm3, p = 0.005) in
blood. HPeV infections were associated with irritability (50% vs.
20%, p = 0.001), clinical sepsis (33% vs. 6%, p = 0.0001), antibiotic
treatment (100% vs. 70%, p = 0.002), and PICU admission (30% vs.
9%, p = 0.003). Both groups had similar proportion of fever symp-
toms (93% vs. 100%) and exanthema (18% vs. 14%). Only 2 patients
had sequelae (1 EV and 1 HPeV-positive).
Conclusions: Significant differences in clinical and epidemi-
ological data were observed between EV and HPeV infections.
Different EV types were associated with meningitis (with pleo-
cytosis and leukocytosis) in young children while HPeV type 3
caused more frequently clinical sepsis exclusively in infants less
than 2 months of age. Initially the process seems to be more severe
in HPeV-infected children, maybe due to the shorter age of the
patients, although prognosis is good in general. EVs and HPeVs
should be included in the routine screening of samples from young
children with neurological or systemic infections to improve their
clinical management, preventing unnecessary treatment and pro-
longed hospitalization.
Abstract no: 139
Presentation at ESCV 2016: Poster 233
Evaluation of viral etiology in central nervous
system infections for seven years
A. Zeytinoglu ∗, S. Erensoy , R. Sertoz ,
I. Altuglu , C. Cicek , M. Kayin , H. Sirin ,
S. Taner
1 Ege University Faculty of Medicine, Dept of Medical
Microbiology, Izmir, Turkey
2 Ege University Faculty of Medicine, Dept of
Neurology, Izmir, Turkey
3 Ege University Faculty of Medicine, Dept of Public
Health, Izmir, Turkey
The serious diseases of the central nervous system (CNS),
encephalitis and meningitis, has a high fatality and sequele rate
especially if it is not diagnosed and treated. Molecular methods of
detection, especially PCR, are the tool of choice for viral diagnosis in
CNS infections. In this study, viral etiological agents were evaluated
in CNS infections suspected with viral etiology in total of routine
3778 tests, done from cerebrospinal fluid (CSF) samples.
Materials andmethods:Cerebrospinal fluid samples of patients
suspected of viral CNS infections that were admitted to our
laboratory between 1.1.2009 and 31.12.2015 for HSV1, HSV2,
VZV, EBV, CMV, HHV6 and enterovirus (EV) were evaluated.